Hey everyone here's the latest update from our lab team!!!!

The team is currently doing the 3A Assembly which consists of  assembling 2 biobrick parts together. In this assembly different enzymes are used for creating different flanking sequences. All the 3parts are present in different antibiotic resistant plasmids resulting in small and effective protocol.3A assembly for S0x will take place in 3 stages. Here,  the ligated parts of previous assembly will be implemented as part A always.

In stage 1 part A= promoter, part B= SQR gene and pSB1K3 plasmid backbone.

In succeeding stage the ligated part(promoter+SQR) will act as part A, RBS as part B and pSB1T3 as plasmid backbone.

Last stage will contain promter+SQR+RBS as part A , Cys1 gene as part B and pSB1A3 plasmid backbone.

Out of the 3 levels of this assembly, they have successfully completed the first level for SOx coli where they ligated the promoter and SQR gene and transformed this plasmid to the E coli strain. The team is now testing the clone obtained at the end of the first level to ensure that the ligation took place properly and it is not the original plasmid that's getting transformed. The team plans to perform these levels thrice to ensure accurate results.


In case of the NOx coli, the team has  managed to successfully carry out ligation and is working on transformation of the plasmid. 

Also, efforts are being made  on isolating Linearised Plasmid Backbone from different biobricks given in the kit. As you can imagine, there is steady progress going on in our lab and hopefully by the time you need the next update we should have more good news to report!!!!

-Aashika Sekar & Rishabh Mathur